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中国·银河集团-WWW.9873.cσm|官方网站是国内ELISA试剂盒优质供应商,代理销售不同ELISA试剂盒品牌的进口/国产ELISA试剂盒,专业供应科研实验所需的培养基,抗体,动物血清血浆,标准品对照品,化学试剂,酶联免疫试剂盒,白介素试剂盒,金标检测试剂盒,微生物,蛋白质,ELISA种属涵盖广,凭借多年行业经验,完善的售后服务,高质量的产品。
沪鼎产品文献:植物LOX,PLD,ELISA试剂盒引用文献引用文献
【文献标题】High-pressure carbon dioxide treatment and vacuum packaging alleviate
the yellowing of peeled Chinese water chestnut (Eleocharis tuberosa)
【作者】Xuan Zhou,Xiaoyun Xu,Ayesha Murtaza,et.al
【作者单位】华中农业大学(Huazhong Agricultural Universit)
【文献中引用产品】
植物磷脂酶D(PLD)ELISA试剂盒
植物脂肪氧化酶(LOX)ELISA试剂盒 活性
【关键词】Chinese Water Chestnut, Eleocharis tuberosa ,Yellowing ,High-pressure carbon dioxide, Vacuum packaging
【影响因子(IF)】8.74
【出版期刊】《Food Packaging and Shelf Life》
【产品原文引用】Phospholipase D (PLD) and lipid oxidase (LOX) activity. The
crude extracted enzyme solution was used to determine PLD and LOX
activities by following the instruction given in ELISA kit (Shanghai
Huding Biological Technology Co., Ltd.). The standard curve was
demonstrated using the standard concentration as the horizontal coordinate and the OD value as the vertical coordinate.
沪鼎产品文献:斑马鱼卵黄原蛋白(VTG)ELISA试剂盒引用文献
【文献标题】Microcystin-LR influences the in vitro oocyte maturation of zebrafish by activating the MAPK pathway
【作者】Wanjing Liu, Chunhua Zhan, Tongzhou Zhang,et.al
【作者单位】华中农业大学(Huazhong Agricultural University)
【文献中引用产品】
斑马鱼卵黄原蛋白(VTG)ELISA试剂盒
【关键词】Microcystin,Meiosis,Oocyte maturation,Hyper-phosphorylation,MAPK
【影响因子(IF)】7.2
【出版期刊】《Aquatic Toxicology》
【产品原文引用】
PP2A and MPF enzymatic activity and VTG content
PP2A enzymatic activity was assayed according to the instruction of Serine/Threonine Phosphatase Assay System (Promega, V2460;Madison, Wisconsin, USA). Live oocytes were rinsed and homogenized
in ice-cold PSB to obtain homogenate. The homogenate was added into the provided spin columns with Sephadex beads to remove endogenous phosphates. The supernatant was taken for detecting total soluble protein concentration using a bicinchoninic acid assay (BCA kit;Beyotime, Shanghai,China). The reaction was initiated by adding 15 μL of reaction premixes containing 5 μL of 1 mM phosphopeptide and 10 μL of PPase-2A 5×reaction buffer. After reaction at 37 °C for 30 min,50 μL of stop solution was added to each well. The data was read at 630 nm. The activity levels of MPF and the contents of VTG were determined through the zebrafish MPF ELISA Kit (Mlbio, Shanghai, China) and the zebrafish VTG ELISA Kit (Huding, Shanghai, China), following the manufacturer’sprotocol.
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