中国·银河集团-WWW.9873.cσm|官方网站是国内ELISA试剂盒优质供应商，代理销售不同ELISA试剂盒品牌的进口/国产ELISA试剂盒，专业供应科研实验所需的培养基，抗体，动物血清血浆，标准品对照品，化学试剂，酶联免疫试剂盒，白介素试剂盒，金标检测试剂盒，微生物，蛋白质，ELISA种属涵盖广，凭借多年行业经验，完善的售后服务，高质量的产品。

IF=3.6

【文献标题】Effect of erythropoietin combined with hypothermia on serum tau protein levels and neurodevelopmental outcome in neonates with hypoxic-ischemic encephalopathy

【作者】Hong-yan Lv，Su-jing Wu，Qiu-li Wang，et.al

【作者单位】邯郸市邯郸妇幼保健院（Handan Maternal and Child Health Care Hospital, Handan）

【文献中引用产品】

人Tau蛋白(TAU)ELISA试剂盒

【关键词】nerve regeneration; erythropoietin; hypothermia; hypoxic-ischemic encephalopathy; neonate; tau protein; biomarkers; prognosis; neuroprotection; neural regeneration

【DOI】doi.org/10.4103/1673-5374.217338

【出版期刊】《NEURAL REGENERATION RESEARCH》

【产品原文引用】

Serum tau protein measurement

Blood samples were taken from the radial vein at 24 hours after birth (before EPO treatment) and 4, 8, and 12 days after EPO treatment. Blood samples were placed in a test tube (specifications: 5 mL, No A06277602; Hebi City Zhong Xing Medical Supplies, Hebi, China), maintained without stirring for 30 minutes at room temperature, and then centrifuged in a L-400 low-speed automatic balancing centrifuge (Hunan Xiangyi Laboratory Instrument Development, Changsha, Hunan Province, China) at 3,000 r/min for 15 minutes.Serum was removed into another test tube and placed in a freezer at ?70°C until use. To avoid any influence on the results, blood transfusions to the neonates were avoided before collecting blood samples. Serum tau protein was detected using an enzyme-linked immunosorbent assay. The kit was provided by the Shanghai HuDing Biological Science and Technology Co., Ltd.

IF=7.2

【文献标题】Microcystin-LR influences the in vitro oocyte maturation of zebrafish by activating the MAPK pathway

【作者】Wanjing Liu, Chunhua Zhan, Tongzhou Zhang,et.al

【作者单位】华中农业大学（Huazhong Agricultural University）

【文献中引用产品】

斑马鱼卵黄原蛋白（VTG）ELISA试剂盒

【关键词】Microcystin，Meiosis，Oocyte maturation，Hyper-phosphorylation，MAPK

【DOI】doi.org/10.1016/j.aquatox.2019.105261

【出版期刊】《Aquatic Toxicology》

【产品原文引用】

PP2A and MPF enzymatic activity and VTG content

PP2A enzymatic activity was assayed according to the instruction of Serine/Threonine Phosphatase Assay System (Promega, V2460;Madison, Wisconsin, USA). Live oocytes were rinsed and homogenized

in ice-cold PSB to obtain homogenate. The homogenate was added into the provided spin columns with Sephadex beads to remove endogenous phosphates. The supernatant was taken for detecting total soluble protein concentration using a bicinchoninic acid assay (BCA kit;Beyotime, Shanghai,China). The reaction was initiated by adding 15 μL of reaction premixes containing 5 μL of 1 mM phosphopeptide and 10 μL of PPase-2A 5×reaction buffer. After reaction at 37 °C for 30 min,50 μL of stop solution was added to each well. The data was read at 630 nm. The activity levels of MPF and the contents of VTG were determined through the zebrafish MPF ELISA Kit (Mlbio, Shanghai, China) and the zebrafish VTG ELISA Kit (Huding, Shanghai, China), following the manufacturer’s protocol.

IF=9.5

【文献标题】Alogliptin prevents diastolic dysfunction and preserves left ventricular mitochondrial function in diabetic rabbits

【作者】Xiaowei Zhang,Zhiwei Zhang,Yajuan Yang,et.al

【作者单位】天津医科大学第二医院(Second Hospital of Tianjin Medical University)

【文献中引用产品】

兔胰高血糖素（Glucagon）ELISA试剂盒

兔8羟基脱氧鸟苷（8-OHdG）ELISA试剂盒

【关键词】Dipeptidyl peptidase-4 inhibitors, Diabetic cardiomyopathy, Diabetes mellitus, Mitochondrial function, Mitochondrial biogenesis

【DOI】doi.org/10.1186/s12933-018-0803-z

【出版期刊】《BMC BIOLOGY》

【产品原文引用】

Serum biochemical, infammatory oxidative stress measurements 

Blood samples collected were used for serum biochemical examination, including fasting glucose, total cholesterol, triglyceride, low-density lipoprotein cholesterol (LDL-c), high density lipoprotein cholesterol (HDL-c) and creatinine level by full automatic biochemical analyzer. Fasting insulin and GLP-1 level were assessed using Rabbit Insulin ELISA Kit (Wuhan Huamei Biological Engineering Co., Ltd, Hubei Province, China) and Rabbit Glucagon Like Peptide-1 ELISA Kit (Shanghai Huding Biological Technology Co., Shanghai, China) according to the manufacturer’s instructions respectively. Serum oxidative stress related markers Lipid Peroxidation Malondialdehyde (MDA) and 8-hydroxy-2 deoxyguanosine (8-OHdG) were assessed by MDA Assay Kit (Nanjing Jianchen Bioengineering Institute, Jiangsu Province, China) and Rabbit 8-OHdG ELISA Kit (Shanghai Huding Biological Technology Co., Shanghai, China). Serum antioxidant enzyme superoxide Jianchen Bioengineering Institute, Jiangsu Province, China). And the infammation maker high-sensitivity C-reactive protein (hs-CRP) level was detected using Rabbit hs-CRP ELISA KIT (Wuhan Huamei Biological Engineering Co., Ltd, Hubei Province, China).

IF=5.9

【文献标题】Rapeseed protein-derived ACE inhibitory peptides LY, RALP and GHS show

antioxidant and anti-inflammatory effects on spontaneously hypertensive rats

【作者】Rong He，Yujiao Wang，Yijie Yang，et.al

【作者单位】南京财经大学（Nanjing University of Finance and Economics）

【文献中引用产品】

人白细胞介素6(IL-6)ELISA试剂盒

人肿瘤坏死因子α(TNF-α)ELISA试剂盒

人诱导型一氧化氮合成酶(iNOS)ELISA试剂盒

【关键词】Rapeseed peptide，ACE inhibition，Anti-inflammatory effect，Cytokine，Oxidative  stress

【DOI】doi.org/10.1016/j.jff.2019.02.031

【出版期刊】《Journal of Functional Foods》

【产品原文引用】

Materials and reagents

RAW264.7 cells were obtained from the cell bank of the Chinese Academy of Sciences(Shanghai,China). LPS from Escherichia coli O55:B5,3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide(MTT), fetal bovine serum, and hematoxylin and eosin (H&E) solutions were purchased from Sigma-Aldrich Trading Co., Ltd. (Shanghai,China). The SOD Assay Kit, Malondialdehyde (MDA) Assay Kit, Nitric Oxide (NO) Assay Kit and Nitric Oxide Synthase (NOS) Assay Kit were purchased from Jiancheng Bioengineering Institute (Nanjing, China).The Human Interleukin 6 (IL-6) ELISA Kit, Tumor Necrosis Factor-α(TNF-α) ELISA Kit and Inducible Nitric Oxide Synthase (iNOS) Assay Kit were purchased from Shanghai HuDing Biological Technology Co., Ltd.(Shanghai, China). Phosphate-buffered saline (PBS, pH 7.4) was purchased from Solarbio Science and Technology Co., Ltd. (Beijing, China).All other chemical reagents were of analytical grade. The ACE inhibitory peptides Leu-Tyr (LY), Arg-Ala-Leu-Pro (RALP) and Gly-His-Ser(GHS) were synthesized with purity over 95% by Synthgene Biological Technology Co., Ltd. (Shanghai, China).