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日期:2022-11-30浏览:463次
植物铁氧还蛋白-NADP氧化还原酶(FNR)活性引用文献
【文献标题】Positive effects of NaCl on the photoreaction and carbon assimilation efficiency in uaeda salsa
【作者】Qiang Li, Ru Liu, Zihan Li, Hai Fanet.al
【作者单位】山东师范大学 Sangdong Normal University
【文献中引用产品】
植物铁氧还蛋白-NADP氧化还原酶(FNR)活性
【关键词】Abbreviations: Electron transport rate, ETR; Ferredoxin-NADP oxidoreductase, FNR; Ribulose-1 5-bisphosphate carboxylase, Rubisco; Rubisco activase, RCA.
【影响因子(IF)】4.2
【出版期刊】《Positive effects of NaCl on the photoreaction and carbon assimilation efficiency in uaeda salsa》
【产品原文引用】Determination of the activities of ferredoxin-NADP oxidoreductase, ribulose-1,5-bisphosphate arboxylase and Rubisco activase A total of 0.3 g of mature leaves were placed in a pre-cooled mortar, and the leaves were ground on ice with chilled 50 mM Tris buffer at pH 7.4. The supernatant was extracted by centrifugation and stored at -20 °C. The activity of FNR was assayed using a plant ELISA kit (Shanghai Huding Biotechnology Co., Ltd., Shanghai, China). A microplate reader (Spectra Max M5; Molecular Devices, San Jose, CA, USA) was used to assay the activity of the enzyme according to the manufacturer’s instructions (Zhao et al., 2018). Three replicates were established for each concentration of NaCl.The activities of Rubisco and RCA were assayed as described for FNR.
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