【文献标题】Replacing fish oil with palm oil: Effects on mRNA expression of fatty acid transport genes and signalling factors related to lipid metabolism in Nile tilapia (Oreochromis niloticus)

【作者】Christian Larbi Ayisi,Jin-Liang Zhao,Xue-Ming Hua,et.al

【作者单位】上海海洋大学（Shanghai Ocean University）

【文献中引用产品】

鱼过氧化物酶体增殖物激活受体α(PPAR-α)ELISA试剂盒

鱼过氧化物酶体增殖物激活受体β(PPARβ)ELISA试剂盒

鱼脂蛋白酯酶（LPL）ELISA试剂盒

鱼脂肪酸结合蛋白3（FABP3）ELISA试剂盒

鱼金属忍耐蛋白(MTP)ELISA试剂盒

鱼可溶性肝X受体(LXR)ELISA试剂盒

鱼脂肪酸延长酶5(ELOVL5)ELISA试剂盒

鱼葡萄糖6磷酸脱氢酶(G6PD)ELISA试剂盒

鱼苹果酸酶（ME）ELISA试剂盒

【关键词】ELOVL 5, fatty acid metabolism, fatty acid transport, glucose 6-phosphate dehydrogenase, 

lipoprotein lipase, palm oil

【DOI】doi.org/10.1111/anu.12821

【出版期刊】《Aquaculture Nutrition》

【产品原文引用】

Enzyme activities

Enzyme-linked immunosorbent assay (ELISA) was used to measure the enzyme activities of PPAR-α, PPAR-β, LPL, FABP-3, MTP, LXR, ELOVL 5, 6GPD, G6PD and ME with a commercial kit from Shanghai Ding Biological Technology Company Limited (Shanghai, China). A ground glass homogenizer was used to homogenize liver (0.5-1.0 g) on ice. The homogenates were centrifuged (110 000 g, 20 min at 4°C), and the clear phase between the top layer and the pellets was used for the analysis. At 450 nm, an ELISA microplate reader was used to measure the optical density (OD) of samples in a 96-well plate. According to the manufacturer’s instructions, a standard curve was generated and the standard diversity calculated using Excel 2003. Enzyme activity units (IU), defined as moles of substrate, were converted into product per minute at assay temperature.